BIOTECHOLOGY
PAPER 1
(THEORY)
(Three hours)
Part I
Question 1
(a) Mention any one significant difference between each of the following:
(i) Western bottling and southern bottling.
(ii) Pyranoses and furanoses
(iii) RNA polymerase and DNA polymerase.
(iv) Primary and secondary culture.
(v) Codon and anticodon.
(b) Answer the following questions:
(i) What is optical isomerism?
(ii) What are termination codons?
(iii) What is meant by central dogma?
(iv) Who discovered enzyme restriction endonucleases?
(v) Why is Agrobacterium tumifaciens called as the natural genetic engineer of plants?
(c) Write the full form of the following:
(i) MCS
(ii) NBPGR
(iii) AFLP
(iv) SCP
(v) EST
(d) Explain briefly:
(i) Prosthetic group
(ii) Synthetic seeds
(iii) Palindromic sequences
(iv) Restriction sites
(v) Single strand binding proteins
PART II
Answer any five questions.
Question 2
(a) What are carbohydrates? Classify them giving examples. [
(b) Explain the steps involved in the post translational modification of proteins.
(c) Enlist four characteristics of a genetic code.
Question 3
(a) Explain the following biochemical techniques:
(i) Electrophoresis
(ii) Centrifugation.
(b) Explain the different methods of freeze preservation of germplasm.
(c) What is meant by the term insertional inactivation?
Please refer to attached file for ISC Question Papers 2013 Biotechnology