CBSE Class 12 Biology Biotechnology Principles and Processes MCQs Set 04

Question : The restriction enzymes are used in genetic engineering, because 
a) they can degrade harmful proteins
b) they can join different DNA fragments
c) they can cut DNA at spe cific base sequence
d) they are nucleases that cut DNA at variable sites

Answer :  A

Question : The basis for DNA fingerprinting is  
a) occurrence of Restriction Fragment Length Polymorphism (RFLP)
b) phe noty pic   differences  between individuals
c) availability of cloned DNA
d) knowledge of human karyotype

Answer :  A

Question : Introduction of one or more genes into an organism which normally does not possess them or their deletion by using artificial means (not by breeding) comes under 
a) Molecular Biology
b) Cytogenetics
c) Genetic hybridization
d) Genetic Engineering

Answer :  D

Question : Recombinant DNA is obtained by cleaving the pro-DNA by                                   
a) primase
b) exonucleases
c) ligase
d) restriction endonuclease

Answer :  D

Question : Genetic engineering is possible, because
a) the phenomenon of transduction in bacteria is well understood
b) we can see DNA by electron microscope
c) we can cut DNA at specific sites by endonucleases like DNAse-I
d) restriction endonucleases purified from bacteria can be used in vitro

Answer :  D

Question : The process of replication in plasmid DNA, other than initiation, is controlled by 
a) mitochondrial gene
b) bacterial gene
c) plasmid gene   
d) None of the above

Answer :  B

Question : Which of the following is related to genetic engineering?               
a) Mutation             
b)  Plasmid
c) Plastid               
d) Heterosis

Answer :  B

Question : Plasmids are suitable vectors for gene clon- ing because  
a) these are small circular DNA molecules which can integrate with host chromo- somal DNA
b) these are small circular DNA molecules with their own replication origin site
c) these can shuttle between prokaryotic and eukaryotic cells
d) these often carry antibiotic resistance genes 

Answer :  B

Question : Maximum number of bases in plasmids dis- covered so far is            
a) 50 kilo base       
b)  500 kilo base
c) 5000 kilo base   
d) 5 kilo base

Answer :  B

Question : Plasmid is                                                 
a) fragment of DNA which acts as vector
b) a fragment which joins two genes
c) mRNA which acts as carrier
d) autotrophic fragment

Answer :  A

Question : In bacteria, plasmid is                            
a) extrachromosomal material
b) main DNA
c) non-functional DNA
d) repetetive gene

 Answer :  A

Question : DNA finger-printing refers to                     
a) molecular analysis or profiles of DNA samples
b) analysis of DNA samples using imprint- ing device
c) techniques used for molecular analysis of different specimens of DNA
d) techniques used for identification of finger prints of individuals

 Answer :  A

Question : Restriction endonudeases                      
a) are present in mammalian cells for degradation of DNA when the cell dies
b) are used in genetic engineering for ligating two DNA molecules
c) are used for in vitro DNA synthesis
d) are synthesized by bacteria as part of their defence mechanism

Answer :  A

Question : Which one of the following is commonly used in transfer of foreign DNA into crop plants?                                                   
a) Agrobacterium tumefaciens
b) Penicillium expansum
c) Trichoderma harzianum
d) Meloidogyne incognita

Answer :  A

Question : Which one of the following is used as vector for cloning genes into higher organisms?
a) Baculovirus                   
b) Salmonella typhimurium
c) Rhizopus nigricans
d) Retrovirus

Answer :  D

Question : DNA or RNA segment tagged with a radio- active molecule is called:            
a) Vector               
b)  Probe
c) Clone               
d) Plasmid

Answer :  B

Question : Restriction endonucleases are enzymes which                                          
a) make cuts at specific positions within the DNA molecule
b) recognize a specific nucleotide sequence for binding of DNA ligase
c) restrict the action of the enzyme DNA polymerase
d) remove nucleotides from the ends of the DNA molecule

Answer :  A

Question : Satellite DNA is useful tool in       
a) Organ transplantation
b) Sex determination
c) Forensic science
d) Genetic engineering

Answer :  D

Question : Silencing of mRNA has been used in pro- ducing transgenic plants resistant to
a) Bacterial blights
b) Bollworms
c) Nematodes
d) White rusts

Answer :  C

Question : Which one of the following techniques made it possible to genetically engineer living or- ganisms                                    
a) Hybridization
b) Recombinant DNA techniques
c) X-ray diffraction
d) Heavier isotope labelling

Answer :  B

Question : There is a restriction endonuclease called EcoRI. What does “co” part in it stand for ? 
a) Colon               
b)  Coelom
c) Coenzyme       
d) Coli  

Answer :  D