CBSE Class 12 Biology Biotechnology Principles and Processes MCQs Set 02

Question. c-DNA probes are copied from the messenger RNA molecules with the help of
(a) restriction enzymes
(b) reverse transcriptase
(c) DNA polyermase
(d) adenosine deaminase

Answer : B

Question. The DNA molecule to which the gene of interest is integrated for cloning is called
(a) template
(b) carrier
(c) transformer
(d) vector.

Answer : D

Question. The introduction of T-DNA into plants involves
(a) exposing the plants to cold for a brief period
(b) allowing the plant roots to stand in water
(c) infection of the plant by Agrobacterium tumefaciens
(d) altering the pH of the soil, then heat-shocking the plants.

Answer : C

Question. Commonly used vectors for human genome sequencing are
(a) T – DNA
(b) BAC and YAC
(c) expression vectors
(d) T/A cloning vectors.

Answer : B

Question. A mutant strain of T4 – Bacteriophage, R-II, fails to lyse the E. coli but when two strains R-IIX and R-IIY are mixed then they lyse the E. coli. What may be the possible reason?
(a) Bacteriophage transforms in wild.
(b) It is not mutated.
(c) Both strains have similar cistrons.
(d) Both strains have different cistrons.

Answer : D

Question. Maximum number of bases in plasmids discovered so far
(a) 50 kilo base
(b) 500 kilo base
(c) 5000 kilo base
(d) 5 kilo base.

Answer : B

Question. There is a restriction endonuclease called EcoRI. What does “co” part in it stand for?
(a) Colon
(b) Coelom
(c) Coenzyme
(d) Coli

Answer : D

Question. In genetic engineering, a DNA segment (gene) of interest, is transferred to the host cell through a vector. Consider the following four agents (i-iv) in this regard and select the correct option about which one or more of these can be used as a vector/vectors.
(i) Bacterium
(ii) Plasmid
(iii) Plasmodium
(iv) Bacteriophage
(a) (i), (ii) & (iv)
(b) (i) only
(c) (i) & (iii)
(d) (ii) & (iv)

Answer : D

Question. Given below are four statements pertaining to separation of DNA fragments using gel electrophoresis. Identify the incorrect statements.
(i) DNA is negatively charged molecule and so it is loaded on gel towards the anode terminal.
(ii) DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA.
(iii) Smaller the size of DNA fragment larger is the distance it travels through it.
(iv) Pure DNA can be visualized directly by exposing UV radiation.
Choose the answer from the options given below.
(a) (i), (iii) and (iv)
(b) (i), (ii) and (iii)
(c) (ii), (iii) and (iv)
(d) (i), (ii) and (iv)

Answer : D

Question. The DNA fragments separated on an agarose gel can be visualised after staining with
(a) acetocarmine
(b) aniline blue
(c) ethidium bromide
(d) bromophenol blue.

Answer : C

Question. Which of the following are used in gene cloning?
(a) Nucleoids
(b) Lomasomes
(c) Mesosomes
(d) Plasmids

Answer : D

Question. Genetic engineering is possible, because
(a) we can cut DNA at specific sites by endonucleases like DNase I
(b) restriction endonucleases purified from bacteria can be used in vitro
(c) the phenomenon of transduction in bacteria is well understood
(d) we can see DNA by electron microscope.

Answer : B

Question. The most thoroughly studied of the known bacteriaplant interactions is the
(a) cyanobacterial symbiosis with some aquatic ferns
(b) gall formation on certain angiosperms by Agrobacterium
(c) nodulation of Sesbania stems by nitrogen fixing bacteria
(d) plant growth stimulation by phosphatesolubilising bacteria.

Answer : B

Question. Human Genome Project (HGP) is closely associated with the rapid development of a new area in biology called as
(a) biotechnology
(b) bioinformatics
(c) biogeography
(d) bioscience

Answer : B

Question. The process of separation and purification of expressed protein before marketing is called
(a) downstream processing
(b) bioprocessing
(c) postproduction processing
(d) upstream processing.

Answer : A

Question. Match the items in column I with their uses in column II and choose the right option.
    Column I                              Column II
A. ELISA                    (i) Antigen-antibody interaction
B. PCR                      (ii) Gene amplification
C. Biolistics               (iii) Direct introduction of recombinant DNA
D. Micro-injection      (iv) Gold coated DNA
(a) A-(i), B-(ii), C-(iv), D-(iii)
(b) A-(ii), B-(i), C-(iv), D-(iii)
(c) A-(iv), B-(i), C-(ii), D-(iii)
(d) A-(i), B-(iv), C- (ii), D-(iii)

Answer : A

Question. Which one of the following is used as vector for cloning genes into higher organisms?
(a) Baculovirus
(b) Salmonella typhimurium
(c) Rhizopus nigricans
(d) Retrovirus

Answer : D

Question. Identify A, B, C and D in the given diagram.

(a) A-ori, B-ampR, C-tetR, D-HindIII
(b) A-HindIII, B-tetR, C-ampR, D-ori
(c) A-ampR, B-tetR, C-HindIII, D-ori
(d) A-tetR, B-HindIII, C-ori, D-ampR

Answer : A

Question. In genetic engineering, the antibiotics are used
(a) as selectable markers
(b) to select healthy vectors
(c) as sequences from where replication starts
(d) to keep the cultures free of infection.

Answer : A

Question. There is a restriction endonuclease called EcoRI. What does “co” part in it stand for?
(a) colon
(b) coelom
(c) coenzyme
(d) coli

Answer : D

Question. What is the first step in the Southern blot technique?
(a) Denaturation of DNA on the gel for hybridization with specific probe.
(b) Production of a group of genetically identical cells.
(c) Digestion of DNA by restriction enzyme.
(d) Denaturation of DNA from a nucleated cell such as the one from the scene of crime.

Answer : D

Question. Which one is a true statement regarding DNA polymerase used in PCR?
(a) It is used to ligate introduced DNA in recipient cells.
(b) It serves as a selectable marker.
(c) It is isolated from a virus.
(d) It remains active at high temperature.

Answer : D

Question. The enzymes which are absolutely necessary for recombinant DNA technology are
(a) restriction endonucleases and topoisomerases
(b) endonucleases and polymerases
(c) restriction endonucleases and ligases
(d) peptidases and ligases.

Answer : C

Question. The DNA molecule to which the gene of interest is integrated for cloning is called
(a) template
(b) carrier
(c) transformer
(d) vector.

Answer : D

Question. DNA precipitation out of a mixture of biomolecules can be achieved by treatment with
(a) chilled chloroform
(b) isopropanol
(c) chilled ethanol
(d) methanol at room temperature.

Answer : C

Question. Restriction endonucleases are used as
(a) molecular build up at nucleotides.
(b) molecular degradation to DNA breakup.
(c) molecular knives for cutting DNA at specific sites.
(d) molecular cement to combine DNA sites.

Answer : C

Question. Which of the following restriction enzymes produces blunt ends?
(a) SalI
(b) EcoRV
(c) XhoI
(d) HindIII

Answer : B

Question. Gel electrophoresis is used for
(a) construction of recombinant DNA by joining with cloning vectors
(b) isolation of DNA molecules
(c) cutting of DNA into fragments
(d) separation of DNA fragments according to their size.

Answer : D

Question. Which one is a true statement regarding DNA polymerase used in PCR?
(a) It is used to ligate introduced DNA in recipient cells.
(b) It serves as a selectable marker.
(c) It is isolated from a virus.
(d) It remains active at high temperature.

Answer : D

Question. Which of the following is not a feature of the plasmids?
(a) Transferable
(b) Single-stranded
(c) Independent replication
(d) Circular structure

Answer : B

Question. The enzymes which are absolutely necessary for recombinant DNA technology are
(a) restriction endonucleases and topoisomerases
(b) endonucleases and polymerases
(c) restriction endonucleases and ligases
(d) peptidases and ligases.

Answer : C

Question. DNA precipitation out of a mixture of biomolecules can be achieved by treatment with
(a) chilled chloroform
(b) isopropanol
(c) chilled ethanol
(d) methanol at room temperature.

Answer : C