CBSE Class 12 Biology Bio Technology and Its Application Question Bank

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Study Material for Class 12 Biology Chapter 12 Biotechnology and Its Application

Class 12 Biology students should refer to the following Pdf for Chapter 12 Biotechnology and Its Application in Class 12. These notes and test paper with questions and answers for Class 12 Biology will be very useful for exams and help you to score good marks

Class 12 Biology Chapter 12 Biotechnology and Its Application

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BIOLOGY
BIO TECHNOLOGY & ITS APPLICATION
 
1. PCR is a powerful diagnostic technique .Justify. 
2. Name the diagnostic test for AIDS when the concentration of HIV is (a) low (b) high.
3. State the principle used in ELISA? 
4. What is GEAC .State its function? 
5. How are transgenic animals made useful in chemical safety testing and studying diseases? 
6. How is use of r DNA better than using traditional methods of agriculture?
7. Name four application of biotechnology 
8. Mention two applications of biotechnology in medicine with examples 
9. What problem was faced in production of insulin by rDNA technology and how was it resolved 
10. What merits do the products made using Biotechnology have ?Give an example of one such product used in treatment.?
11. What are the three critical research areas of Biotechnology
12. How is Biotechnology used to produce bt cotton? Explain 
13. How has the bt toxin been effective against insects?
14. How can biotechnology help in increasing the yield of tobacco 
15. Name a natural genetic engineer and state how it has been used to create pest resistant tobacco? 
16. Insulin produced earlier had problems This has been overcome with rDNA technology. Explain.Why is Insulin not administered orally?.
17. Gene therapy is a better alternative to the other measures taken for ADA deficiency. Why is it a better alternative . What are the other methods used earlier? How can it be done? 
18. GMO’s have been useful to mankind. List about five useful GMO’s created so far. and how they have been useful 
19. “Most of the industrialized nations are rich financially but poor in biodiversity and traditional knowledge.” Justify this statement with an example.How can you as a Biology student help a tribe who is exploited for their traditional knowledge 
20. How would you have used Biotechnology if you had the complete technology in your hands? 
 
BIO TECHNOLOGY & ITS APPLICATION
(ANSWER KEY)
1. Diagnosis at the time when symptoms are not shown(early stage )of AIDS /Cancer
2. PCR,ELISA
3. Antigen-antibody interaction
4. Genetic Engineering Approval Committee,ensure safety and validity of experiments 
5. Made sensitive to chemicals,making them disease models for studying
6. Use of fertilizers ,pesticides minimized,only desirable traits can be selected for breeding,create tailor made plants for industries
7. (I) therapy,(ii) Diagnostics,(iii) Genetically modified crop in agriculture,(iv)Biofortified food,(transgenic animals)
8. Insulin production ,treatment og genetic disorders with gene therapyas in ADA deficiency
9. To assemble mature insulin from Proinsulin,separate production of A and B in E Coli peptide and combined
10. No allergic reactions(Could cause allergy if made using other animals), á-1 antitrypsin in treatment of emphysema
11. Providing the best catalyst in the form of a microbe/enzyme,creating optimal conditions for their action,downstream processing technologies Toxins of Bacillus introduced into cotton using r DNA,the toxin was activated
12. Toxins of Bacillus introduced into cotton using r DNA,the toxin was activated therefore became reisistant to Bollworms
13. The larva ingests the leaves of Bt cotton containing the toxin,the toxin is activated in the gut of the insect by enzymes,which solubilize the toxin, the toxin binds to the lining of epithelial cells create pores cause swelling and death of the insect
(i)cause for tobacco reduction due to infection by parasitise, nematode Meloidegyne incogntia, in roots.
14. RNA interference,Agrobacterium as vectors,formation of complementary RNA double strand specific to nematode DNA,silencing of mRNA of nematode,makes tobacco pest resistant
15. Agrobacterium tumifaciens,By RNA interference,and stopping of nematode protein production,agrobacterium as vectors to introduce genes into tobacco plants which result sin ,formation of complementary RNA double strand specific to nematode DNA, silencing of mRNA of nematode, makes tobacco pest resistant  
16. Obtained from animals caused allergic reactions,Insulin produced using rDNA did not cause allergic reactions as animal proteins were not used here , Insulin being a protein gets digested if taken orally
17. Peramanent cure ,Bone marrow transplantation and enzyme replacement therapy,introducing cDNA of a functional ADA using retrovirus into the stem cells/early embryonic stages
18. Crops tolerant to abiotic stress,reduced reliance on chemicals,reduced post harvest loss,enhanced nutritional value,physiologicsl models,huam lactalbuminproducing cow(any 5)
19. (i)ethical issues
(iii)e.g basmati an Indian variety, patent obtained by US
(iv)awareness of Biopiracy and need for Biopatent of thr traditional resurces and wisdom
20. Any five applications of the students choice-values of societal needs problem awareness, selflessness reflected in the answers
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CBSE Class 12 Biology Chapter 12 Biotechnology and Its Application Study Material

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