CBSE Class 12 Biotechnology Sample Paper 2025 Solved

Section - A

Q. No. 1 to 12 are multiple choice questions. Only one of the choices is correct. Select and write the correct choice as well as the answer to these questions.

1 An approach that utilises analysis of genome present in different environment to screen presence of variety of molecules.
A. Metagenomics
B. Proteomics
C. Strain preservation
D. Mutant selection

Answer : B

2 In animal cell culture, the CO2 levels in the incubator is usually maintained at-
A. 1-2%
B. 3-4%
C. 5-10%
D. 11-15%

Answer : C

3 Even if we know where the genes are in each genome, it’s difficult to count them due to:
(i) splice variants
(ii) overlapping genes
(iii) Post – trancriptional events
(iv) Mutations
A. (i), (iii)
B. (i), (ii)
C. (iii), (iv)
D. (ii), (iv)

Answer : B

4 Avocado growers in Kerela want to find a way to genetically engineer their crop to prevent it from ripening during shipping. This can be achieved by introducing-
A. genes for carotenoid biosynthesis.
B. gene from Bacillus amyloliquefaciens.
C. ethylene forming gene(s).
D. gene for protein containing sulphur rich amino acids.

Answer : C

5 Microbial species used for commercial production of citric acid is-
A. Saccharomyces cerevisiae
B. Aspergillus niger
C. Streptomyces griseus
D. Aspergillus oryzae

Answer : B

6 To reduce the possibility of religation of vector without picking insert, the following method or methods may be used-
(i) Use of DNA Ligase
(ii) Use of Alkaline Phosphatase
(iii) Use of two different Restriction enzymes for cloning
(iv) Hybridoma technology
Select the correct answer from options below
A. (i), (iv)
B. (ii), (iii)
C. (iii), (iv)
D. (ii), (iv)

Answer : D

7 In a comparative cDNA microarray, the cDNA obtained from the normal and diseased cells are labelled with red and green fluorescent dyes respectively. The yellow colour of the spot indicates-
A. Gene is expressed only in normal cells.
B. Gene is not expressed either in normal cells or in diseased cells.
C. Gene expressed only in diseased cells.
D. Gene expressed in equal measure in both types of cells.

Answer : D

8 Which disease has its locus on chromosome no 1 and 6?
A. Huntington's disease
B. Migraine
C. Alzheimer’s disease
D. Cystic fibrosis

Answer : B

9 A molecular disease which leads to increased hydrophobic interactions within the haemoglobin protein is-
A. Thalassemia
B. Sickle Cell anaemia
C. Mad Cow
D. SCID

Answer : B

10 Interleukin-2 produced using CHO cell line is used for-
A. anaemia therapy.
B. cancer therapy.
C. stroke.
D. treatment of autoimmune disease.  

Answer : B

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