CBSE Class 12 Biology Biotechnology and Its Applications MCQs Set 03

Question. Which one of the following is not a GMO?
(a) Bt brinjal
(b) Golden rice
(c) Tracy
(d) Dolly

Answer : D

Question. Maximum number of existing transgenic animals is of
(a) fish
(b) mice
(c) cow
(d) pig.

Answer : B

Question. An improved variety of transgenic basmati rice
(a) does not require chemical fertilizers and growth hormones
(b) gives high yield and is rich in vitamin A
(c) is completely resistant to all insect pests and diseases of paddy
(d) gives high yield but has no characteristic aroma.

Answer : B

Question. ELISA is used to detect viruses where the key reagent is
(a) alkaline phosphatase
(b) catalase
(c) DNA probe
(d) RNase.

Answer : A

Question. Anticoagulant hirudin is found in:
(a) In Snake
(b) In Lizard
(c) In Leech
(d) In Scorpion

Answer : C

Question. GEAC stands for
(a) Genome Engineering Action Committee
(b) Ground Environment Action Committee
(c) Genetic Engineering Approval Committee
(d) Genetic and Environment Approval Committee.

Answer : C

Question. The process of RNA interference (RNAi) has been used in the development of plants resistant to
(a) nematodes
(b) fungi
(c) viruses
(d) insects.

Answer : A

Question. In RNAi, the genes are silenced using
(a) dsRNA
(b) ssDNA
(c) ssRNA
(d) dsDNA.

Answer : A

Question. Transgenic animals are
(a) Animals whose DNA is manipulated to possess and express an extra (foreign) gene.
(b) Animals whose RNA is manipulated to possess and express an extra (foreign) gene.
(c) Animals whose both DNA and RNA are manipulated to possess and express an extra (foreign) gene.
(d) None of the above.

Answer : A

Question. Crystals of Bt toxin produced by some bacteria do not kill the bacteria themselves because
(a) bacteria are resistant to the toxin.
(b) toxin is immature.
(c) toxin is inactive.
(d) bacteria enclose toxin in a special sac.

Answer : C

Question. The plasmid of which one of the following has ‘nif’ gene in it?
(a) Rhizobium
(b) Agrobacterium tumefaciens
(c) Bacillus thuringiensis
(d) Salmonella typhimurium

Answer : A

Question. Which one of the following vectors is used to replace the defective gene in gene therapy?
(a) Adenovirus
(b) Cosmid
(c) Ri plasmid
(d) Ti plasmid

Answer : A

Question. Which kind of therapy was given in 1990 to a four-year-old girl with adenosine deaminase
(ADA) deficiency?
(a) Gene therapy
(b) Chemotherapy
(c) Immunotherapy
(d) Radiation therapy

Answer : A

Question. The first human hormone produced by recombinant DNA technology is
(a) insulin
(b) estrogen
(c) thyroxin
(d) progesterone.

Answer : A

Question. In transgenics, expression of transgene in target tissue is determined by
(a) enhancer
(b) transgene
(c) promoter
(d) reporter.

Answer : D

Question. Which one of the following is now being commercially produced by biotechnological procedures?
(a) Nicotine
(b) Morphine
(c) Quinine
(d) Insulin

Answer : D

Question. The introduction of T-DNA into plants involves
(a) exposing the plants to cold for a brief period
(b) allowing the plant roots to stand in water
(c) infection of the plant by Agrobacterium tumefaciens
(d) altering the pH of the soil, then heatshocking the plants.

Answer : C

Question. Select the wrong statement.
(a) Human insulin is being commercially produced from a transgenic species of Escherichia coli.
(b) Bt toxin genes cryIAc control the corn borer.
(c) Human protein, a-1-antitrypsin is used to treat emphysema.
(d) The first transgenic cow, Rosie, produced alpha lactalbumin, enriched milk.

Answer : B

Question. The genetic defect, adenosine deaminase (ADA) deficiency may be cured permanently by
(a) administering adenosine deaminase through injection
(b) bone marrow transplantation
(c) enzyme replacement therapy
(d) introducing isolated gene from marrow cells producing ADA into the cells at early embryonic stages

Answer : D

Question. The illegal and unlawful development of biomaterials without payment to the inhabitants of their origin is called
(a) biopatent
(b) biotechnology
(c) biowar
(d) biopiracy.

Answer : D

Question. Which Vitamin has been transferred to golden rice?
(a) Vit-B12
(b) Vit-A
(c) Vit-D
(d) Vit-C

Answer : B

Question. Silencing of mRNA has been used in producing transgenic plants resistant to
(a) boll worms
(b) nematodes
(c) white rusts
(d) bacterial blights.

Answer : B

Question. Recombinant DNA technology discovered by:
(a) Hargobind Khoorana
(b) James D. Watsan
(c) S. Kohen and H. Boer
(d) Sulton and Boveri

Answer : C

Question. The plasmid of which one of the following has ‘nif’ gene in it?
(a) Rhizobium
(b) Agrobacterium tumefaciens
(c) Bacillus thuringiensis
(d) Salmonella typhimurium

Answer : A

Question. Transgenic animals are extensively used for all of the following procedures except
(a) bioremediation
(b) chemical safety
(c) vaccine safety
(d) toxicity test.

Answer : A

Question. Which of the following genes do not occur naturally in living organisms?
(a) Bt genes
(b) RNAi genes
(c) Cry genes
(d) Endogenous cytoplasmic defense genes

Answer : D

Question. Golden rice is a promising transgenic crop. When released for cultivation, it will help in
(a) producing a petrol-like fuel from rice
(b) alleviation of vitamin A deficiency
(c) pest resistance
(d) herbicide tolerance.

Answer : B

Question. Which body of the Government of India regulates GM research and safety of introducing GM organisms for public services?
(a) Genetic Engineering Approval Committee
(b) Research Committee on Genetic Manipulation
(c) Bio-safety committee
(d) Indian Council of Agricultural Research

Answer : A

Question. Basic principle of developing transgenic plants and animals is to introduce the gene of interest into the nucleus of
(a) somatic cell
(b) vegetative cell
(c) germ cell
(d) body cell.

Answer : C

Question. Protein encoded by gene cryIAb controls the infestation of which of the following insects?
(a) Cotton bollworm
(b) Anopheles mosquito
(c) Corn borer
(d) Aedes mosquito

Answer : C

Question. The two polypeptides of human insulin are linked together by
(a) covalent bond
(b) disulphide bridges
(c) hydrogen bonds
(d) phosphodiester bond.

Answer : B

Question. The polio vaccine was tested on :
(a) Mice
(b) Monkey
(c) Rabbit
(d) Dog

Answer : A

Question. The first clinical gene therapy was given in 1990 to a 4 years old girl with enzyme deficiency of
(a) Adenosine deaminase (ADA)
(b) Tyrosine oxidase
(c) Monamine oxidase
(d) Glutamate dehydrogenase

Answer : A